尹斌成，教授，博士生导师。研究方向：智能生物传感系统设计及应用。主要研究内容：(1)基于CRISPR-Cas系统的生物活性分子的可视化及生物调控研究，开发新型的Cas酶级联信号扩增机制，设计多种荧光传感器及信号处理元件，应用于活细胞胞内分子高时空分辨成像；(2)智能化DNA分子机器网络交互系统的构建及应用，构建具有传感、记忆及信号增强的分子机器，开展细胞内多靶标的荧光成像，目的基因的精细调控，为细胞内疾病标志物的动态监测、基因调控网络重构及调节、肿瘤治疗等提供研究方法；(3)发展细胞膜界面修饰及核酸示踪技术，构建可控性多功能外泌体载体对细胞的信号递送，解析其在细胞间信号转递机制，并进一步构建靶向性肿瘤治疗的工程化天然载体，为疾病相关性、临床诊断及治疗等基础及应用研究提供高效工具。

近年来在J Am Chem Soc，Angew Chem Int Ed，Proc Natl Acad Sci USA，Chem Sci，Anal Chem，Chem Commun，Biosens & Bioelectron等国际性刊物上发表SCI收录论文70余篇，SCI论文他引2600余次，3篇ESI高引用论文。申请中国发明专利7项，其中授权4项，撰写英文专著1部。主持和作为主要成员承担了国家自然科学基金（青年、面上、优青）、国家自然科学基金重点项目，国家863项目、上海市自然科学基金、上海市纳米专项等项目。曾获上海市技术发明奖二等奖，高等学校科学研究优秀成果奖（自然科学奖）二等奖，明治乳业生命科学奖，上海市研究生优秀成果（学位论文）等奖项。

电子邮箱：binchengyin@ecust.edu.cn

近10年发表论著：

1. Ting Wang, Yong Liu, Huan-Huan Sun, Bin-Cheng Yin*, and Bang-Ce Ye*. An RNA-Guided Cas9 Nickase-Based Method for Universal Isothermal DNA Amplification. Angewandte Chemie International Edition 58, 5382-5386, 2019.

2. Mei-Ling Gao, Bin-Cheng Yin*, and Bang-Ce Ye*.Construction of a DNA-AuNP-based Satellite Network for Exosome Analysis.Analyst 144, 5996-6003, 2019.

3. Cui-Fang Ning, Ya-Fei Tian, Wen Zhou, Bin-Cheng Yin*, and Bang-Ce Ye*. Ultrasensitive SERS Detection of Specific Oligonucleotides based on Au@AgAg Bimetallic Nanorods. Analyst 144, 2929-2935, 2019.

4. Mei-Ling Gao, Fang He, Bin-Cheng Yin*, and Bang-Ce Ye.A Dual Signal Amplification Method for Exosome Detection based on DNA Dendrimer Self-assembly.Analyst 144, 1995-2002, 2019.

5. Di You, Miao-Miao Wang, Bin-Cheng Yin, Bang-Ce Ye*. Precursor supply for erythromycin biosynthesis: engineering of propionate assimilation pathway based on propionylation modification. ACS Synthetic Biology 8, 371-380, 2019.

6. Di You, Ying Xu, Bin-Cheng Yin, Bang-Ce Ye*. Nitrogen regulator GlnR controls redox sensing and lipids anabolism by directly activating the whiB3 in Mycobacterium smegmatis. Frontiers in Microbiology 10, 74, 2019.

7. Shengyan Chen, Wenping Wei, Bin-Cheng Yin, Yanbin Tong, Jianjiang Lu, Bang-Ce Ye.Development of a highly sensitive whole-cell biosensors for arsenite detection through engineered promoter modifications. ACS Synthetic Biology 8(10), 2295-2302, 2019.

8. Yong Liu, Chong-Yang Ren, Wen-Ping Wei, Di You, Bin-Cheng Yin, and Bang-Ce Ye*. A CRISPR-Cas9 strategy for activating the Saccharopolyspora erythraea erythromycin biosynthetic gene cluster with knock-in bidirectional promoters. ACS Synthetic Biology 8(5), 1134-1143,2019.

9. Pei-Qiang Ma, Cheng-Pin Liang, He-Hua Zhang, Bin-Cheng Yin,* and Bang-Ce Ye. A Highly Integrated DNA Nanomachine Operating in Living Cells Powered by Endogenous Stimulus. Chemical Science 9, 3299-3304, 2018.

10. Fang He, Jing Wang, Bin-Cheng Yin,* and Bang-Ce Ye*. Quantification of Exosome Based on a Copper-Mediated Signal Amplification Strategy. Analytical Chemistry, 90(13), 8072-8079, 2018.

11. Ya-Fei Tian, Cui-Fang Ning, Fang He, Bin-Cheng Yin*, and Bang-Ce Ye*.Highly sensitive detection of exosomes by SERS using gold nanostar@Raman reporter@nanoshell structures modified with a bivalent cholesterol-labeled DNA anchor. Analyst 143, 4915-4922, 2018.

12. Zhi Yuan Mao,Lu Ning Zhu,Jie Gao,Jia Jun Liu,Yin Hui Wei,Xin Yu Li,Bin-Cheng Yin,*andJian Wang*. A CoOOH Nanoflake-based Light Scattering Probe for the Simple and Selective Detection of Uric Acid in Human Serum. Analytical Methods 10, 4951-4957, 2018.

13. Yin Hui Wei, Xin Yu Li, Jie Gao, Jia Jun Liu, Dan Yuan, Bin-Cheng Yin,* and Jian Wang*. Size-dependent Modulation of CoOOH Nanoflakes Light Scattering for Rapid and Selective Detection of Tetracycline in Milk. Journal of Analysis and Testing, 2, 332-341, 2018.

14. Yun Lv, Dan Cheng, Dongdong Su, Mei Chen, Bin-Cheng Yin, Lin Yuan,* and Xiao-Bing Zhang. Visualization of Oxidative Injury in the Mouse Kidney using Selective Superoxide Anion Fluorescent Probes. Chemical Science 9, 7606-7613, 2018.

15. Cheng-Pin Liang, Pei-Qiang Ma,Hui Liu, Xinggang Guo, Bin-Cheng Yin,* and Bang-Ce Ye. Rational Engineering of Dynamic, Entropy-Driven DNA Nanomachine for Intracellular MicroRNA Imaging. Angewandte Chemie International Edition 56 (31), 9077-9081, 2017.

16. Fang He, Hui Liu, Xinggang Guo, Bin-Cheng Yin,* and Bang-Ce Ye. Direct Exosome Quantification via Bivalent-Cholesterol-Labeled DNA Anchor for Signal Amplification. Analytical Chemistry 89 (23), 12968-12975, 2017.

17. Wen Zhou, Ya-Fei Tian, Bin-Cheng Yin,* and Bang-Ce Ye*.Simultaneous Surface-Enhanced Raman Spectroscopy Detection of Multiplexed MicroRNA Biomarkers. Analytical Chemistry 89 (11), 6120-6128, 2017.

18. Jin-Liang Ma, Bin-Cheng Yin,* Xin Wu, and Bang-Ce Ye. Simple and Cost-Effective Glucose Detection based on Carbon Nanodots Supported on Silver Nanoparticles. Analytical Chemistry 89 (2), 1323-1328, 2017.

19. Wen Zhou, Bin-Cheng Yin,* and Bang-Ce Ye*. Highly Sensitive Surface-Enhanced Raman Scattering Detection of Hexavalent Chromium based on Hollow Sea Urchin-Like TiO₂@Ag Nanoparticle Substrate. Biosensors & Bioelectronics 87, 187-194, 2017.

20. Hong-Ya Wang, Jin-Liang Ma, Bin-Cheng Yin,* and Bang-Ce Ye*. Nicotinamide Adenine Dinucleotide Detection based on Silver Nanoclusters Stabilized by a Dumbbell-Shaped DNA Template. Analyst 142, 1765-1771, 2017.

21. Ya-Fei Tian, Wen Zhou, Bin-Cheng Yin,* and Bang-Ce Ye. Highly Sensitive Surface-enhanced Raman Scattering Detection of Adenosine Triphosphate based on Core-satellite Nanoassemblies. Analytical Methods 9, 6038-6043, 2017.

22. Dan Cheng, Yue Pan, Bin-Cheng Yin, Lin Yuan*, Xiao-Bing Zhang. A New Fluorescent Probe with Ultralow Background Fluorescence for Imaging of Endogenous Cellular Selenol under Oxidative Stress. Chinese Chemical Letters 28(10), 1987-1990, 2017.

23. Jin-Liang Ma, Bin-Cheng Yin,* Xin Wu, and Bang-Ce Ye*. Copper-Mediated DNA-Scaffolded Silver Nanocluster On-Off Switch for Detection of Pyrophosphate and Alkaline Phosphatase.Analytical Chemistry 88 (18), 9219-9225, 2016.

24. Ru-Dong Li, Bin-Cheng Yin,* and Bang-Ce Ye*. Ultrasensitive, Colorimetric Detection of MicroRNAs based on Isothermal Exponential Amplification Reaction-Assisted Gold Nanoparticle Amplification.Biosensors & Bioelectronics 86, 1011-1016, 2016.

25. Di You, Bin-Cheng Yin, Zhi-Hai Li, Ying Zhou, Wen-Bang Yu, Peng Zuo, and Bang-Ce Ye*. Sirtuin-dependent reversible lysine acetylation of glutamine synthetases reveals an autofeedback loop in nitrogen metabolism. Proceedings of the National Academy of Sciences of the United States of America 113(24), 6653-6658, 2016.

26. Qian Wang, Bin-Cheng Yin,* and Bang-Ce Ye*.A Novel Polydopamine-based Chemiluminescence Resonance Energy Transfer Method for MicroRNA Detection Coupling Duplex-Specific Nuclease-Aided Target Recycling Strategy. Biosensors & Bioelectronics 80, 366-372, 2016.

27. Jin-Liang Ma, Bin-Cheng Yin,* and Bang-Ce Ye*. A Versatile Proximity-Dependent Probe based on Light-Up DNA-Scaffolded Silver Nanoclusters.Analyst 141, 1301-1306, 2016.

28. Ru-Dong Li, Qian Wang, Bin-Cheng Yin,* and Bang-Ce Ye*. Enzyme-free Detection of Sequence-Specific MicroRNAs based on Nanoparticle-Assisted Signal Amplification Strategy. Biosensors & Bioelectronics 77, 995-1000, 2016.

29. Le Ying, Nuli Xie, Yanjing Yang, Xiaohai Yang, Qifeng Zhou, Bin-Cheng Yin, Jin Huang,* and Kemin Wang*. A Cell-Surface-Anchored Ratiometric I-Motif Sensor for Extracellular pH Detection. Chemical Communications 52, 7818-7821, 2016.

30. Jin-Liang Ma, Bin-Cheng Yin,* and Bang-Ce Ye*.DNA Template-Regulated Intergrowth of Fluorescent Silver Nanocluster Emitter Pair. RSC Advances 5, 98467-98471, 2015.

31. Qian Wang, Ru-Dong Li, Bin-Cheng Yin,* and Bang-Ce Ye*. Colorimetric Detection for Specific MicroRNA Based on Duplex-Specific Nuclease-Assisted Nanoparticle Amplification. Analyst 140, 6306-6312, 2015.

32. Jin-Liang Ma, Bin-Cheng Yin,* and Bang-Ce Ye*. A Novel Linear Molecular Beacon based on DNA-Scaffolded Silver Nanocluster for DNA Detection via Exonuclease III-Assisted Cyclic Amplification. RSC Advances 5, 65437-65443, 2015.

33. Jin-Liang Ma, Bin-Cheng Yin,* Huynh-Nhu Le, and Bang-Ce Ye*. Label-Free Detection of Sequence-Specific DNA based on Fluorescent Silver Nanoclusters-Assisted Surface Plasmon Enhanced Energy Transfer. ACS Applied Materials & Interfaces 7(23), 12856-12863, 2015.

34. Peng-Qiang Leng, Feng-Lan Zhao, Bin-Cheng Yin,* and Bang-Ce Ye*. A Novel, Colorimetric Method for Biogenic Amine Detection based on Arylalkylamine N-Acetyltransferase. Chemical Communications 51, 8712-8714, 2015.

35. Bin-Cheng Yin, Shan Wu, Jin-Liang Ma, and Bang-Ce Ye*. A Novel Molecular Beacon-based Method for Isothermal Detection of Sequence-Specific DNA via T7 RNA Polymerase-Aided Target Regeneration. Biosensors & Bioelectronics 68, 365-370, 2015.

36. Cui-Yuan Yu, Bin-Cheng Yin,* Shilong Wang, Zengguang Xu, and Bang-Ce Ye*. Improved Ligation-Mediated PCR Method Coupled with T7 RNA Polymerase for Sensitive DNA Detection. Analytical Chemistry 86, 7214-7218, 2014.

37. Bin-Cheng Yin,* Jin-Liang Ma, Huynh-Nhu Le, Shilong Wang, Zengguang Xu, and Bang-Ce Ye*. A New Mode to Light Up Adjacent DNA-Scaffolded Silver Probe Pair and Its Application for Specific DNA Detection. Chemical Communications 50 (100), 15991-15994, 2014.

38. Yuan-Chen He, Bin-Cheng Yin,* Lihua Jiang, and Bang-Ce Ye*. Rapid Detection of MicroRNA based on p19-Enhanced Fluorescence Polarization. Chemical Communications 50 (47), 6236-6239, 2014.

39. Xiaoyan Lin, Liang Cui, Yishun Huang, Ya Lin, Yi Xie, Zhi Zhu*, Bin-Cheng Yin, Xi Chen, and Chaoyong James Yang*. Carbon Nanoparticle-Protected Aptamers for Highly Sensitive and Selective Detection of Biomolecules based on Nuclease-Assisted Target Recycling Signal Amplification.Chemical Communications 50, 7646-7648, 2014.

40. Jin Huang, Yong He, Xiaohai Yang, Kemin Wang, Le Ying, Ke Quan, Yanjing Yang, and Bin-Cheng Yin. I-Motif-Based Nano-Flares for Sensing pH Changes in Live Cells. Chemical Communications 50, 15768-15771, 2014.

41. Bin-Cheng Yin,Yu-Qiang Liu, and Bang-Ce Ye*. Sensitive Detection of MicroRNA in Complex Biological Samples via Enzymatic Signal Amplification Using DNA Polymerase Coupled with Nicking Endonuclease.Analytical Chemistry 85 (23), 11487-11493, 2013.

42. Cui-Yuan Yu, Bin-Cheng Yin,* and Bang-Ce Ye*. A Universal Real-Time PCR Assay for Rapid MicroRNA Quantification via the Enhancement of Base-Stacking Hybridization. Chemical Communications 49 (74), 8247-8249, 2013.

43. Min Zhang, Yu-Qiang Liu, Cui-Yuan Yu, Bin-Cheng Yin,* and Bang-Ce Ye*. Multiplexed Detection of MicroRNAs by Tuning DNA-Scaffolded Silver Nanoclusters. Analyst 138 (17), 4812- 4817, 2013.

44. Xin-Ping Wang, Bin-Cheng Yin,* and Bang-Ce Ye*. A Novel Fluorescence Probe of dsDNA-Templated Copper Nanoclusters for Quantitative Detection of MicroRNAs. RSC Advances 3, 8633-8636, 2013.

45. Xin-Ping Wang, Bin-Cheng Yin,* Ping Wang, and Bang-Ce Ye*. Highly Sensitive Detection of MicroRNAs based on Isothermal Exponential Amplification-Assisted Generation of Catalytic G-Quadruplex DNAzyme. Biosensors & Bioelectronics 42, 131-135, 2013.

46. Min Zhang, Huynh-Nhu Le, Xiao-Qin Jiang, Bin-Cheng Yin,and Bang-Ce Ye*.Time-Resolved Probes Based on Guanine/Thymine-Rich DNA-Sensitized Luminescence of Terbium (III). Analytical Chemistry 85 (23), 11665-11674, 2013.

47. Bin-Cheng Yin, Yu-Qiang Liu, and Bang-Ce Ye*. One-step, Multiplexed Fluorescence Detection of microRNAs Based on Duplex-Specific Nuclease Signal Amplification. Journal of the American Chemical Society 134 (11), 5064-5067, 2012.

48. Bin-Cheng Yin, Mingxu You, Weihong Tan, Bang-Ce Ye*. Mercury(II) Ions Detection via Pyrene-Mediated Photolysis of Disulfide Bonds. Chemistry - A European Journal 18(5), 1286-1289, 2012.

49. Bin-Cheng Yin, Bang-Ce Ye*, Hui Wang, Zhi Zhu, Weihong Tan*. Colorimetric Logic Gates based on Aptamer-Crosslinked Hydrogel. Chemical Communications 48, 1248-1250, 2012.

50. Bin-Cheng Yin, Yi-Meng Guan, and Bang-Ce Ye*. An Ultrasensitive Electrochemical DNA Sensor based on the ssDNA-assisted Cascade of Hybridization Reaction. Chemical Communications 48 (35), 4208-4210, 2012.

51. Yu-Qiang Liu, Min Zhang, Bin-Cheng Yin, and Bang-Ce Ye*. Attomolar Ultrasensitive MicroRNA Detection by DNA-Scaffolded Silver-Nanocluster Probe based on Isothermal Amplification. Analytical Chemistry 84(12), 5165-5169, 2012.

52. Di Wu, Bin-Cheng Yin, and Bang-Ce Ye*. A Label-Free Electrochemical DNA Sensor based on Exonuclease III-Aided Target Recycling Strategy for Sequence-Specific Detection of Femtomolar DNA. Biosensors & Bioelectronics 28, 232-238, 2011.

53. Mingxu You, Yan Chen, Lu Peng, Da Han, Bin-Cheng Yin, Bang-Ce Ye, and Weihong Tan*. Engineering DNA Aptamers for Novel Analytical and Biomedical Applications. Chemical Science 2, 1003-1010, 2011.

54. Hui-Jie Tong, Na Jin, Bin-Cheng Yin, and Bang-Ce Ye*. A Novel Separation and Enrichment Method of 17β-Estradiol using Aptamer-Anchored Beads. Bioprocess and Biosystems Engineering 34, 189-195, 2011.

55. Min Zhang, Bin-Cheng Yin, Weihong Tan, and Bang-Ce Ye*. A Versatile Graphene-Based Fluorescence “On/Off” Switch for Multiplex Detection of Various Targets. Biosensors & Bioelectronics 26, 3260-3265, 2011.

56. Min Zhang, Bin-Cheng Yin, Xian-Fei Wang, and Bang-Ce Ye*. Interaction of Peptides with Graphene Oxide and Its Application for Real-Time Monitoring of Protease Activity. Chemical Communications 47, 2399-2401, 2011.

57. Bin-Cheng Yin, Min Zhang, Weihong Tan*, and Bang-Ce Ye*. Peptide-Functionalized Spherical Polyelectrolyte Nanobrushes for Real-Time Sensing of Protease Activity. ChemBioChem 11, 494-497, 2010.

58. Bin-Cheng Yin, Peng Zuo, Hao Huo, Xinhua Zhong, and Bang-Ce Ye*. DNAzyme-Self-Assembled Gold Nanoparticles for Determination of Metal Ions using Fluorescence Anisotropy Assay. Analytical Biochemistry 401, 47-52, 2010.

59. Bin-Cheng Yin, Di Wu, and Bang-Ce Ye*. Sensitive DNA-based Electrochemical Strategy for Trace Bleomycins Detection. Analytical Chemistry 82(19), 8272-8277, 2010.

60. Bin-Cheng Yin, Bang-Ce Ye*, Hui Wang, Weihong Tan*, and Cong-Cong Xie. An Allosteric Dual-DNAzyme Unimolecular Probe for Colorimetric Detection of Copper(II). Journal of the American Chemical Society 131 (41), 14624-14625, 2009.

61. Bin-Cheng Yin, Xian-Fei Wang, and Ye Bang-Ce*. Multiplex Genotyping and Allele Frequency Estimation using Non-Gel Capillary Electrophoresis. Analytical Biochemistry 387, 221-229, 2009.

62. Peng Zuo, Bin-Cheng Yin, Bang-Ce Ye*. DNAzyme-based Microarray for Highly Sensitive Detection of Metal Ions. Biosensors & Bioelectronics 25, 935-939, 2009.

63. Sheng-Quan Jin, Bin-Cheng Yin, and Bang-Ce Ye*. A Multiplexed Bead-based Mesofluidic System for Foodborne Pathogen Bacteria Detection. Applied and Environmental Microbiology 75, 6647-6654, 2009.

64. Bang-Ce Ye*, Yan Zhang, Hui Yu, Wen-Bang Yu, Bao-Hong Liu, Bin-Cheng Yin, Chun-Yun Yin, Yuan-Yuan Li, Ju Chu, Si-Liang Zhang. Time-resolved Transcriptome Analysis of Bacillus subtilis Responding to Valine, Glutamate, and Glutamine. PLoS ONE 4(9), e7073, 2009.

65. Bang-Ce Ye*, Bin-Cheng Yin. Highly Sensitive Detection of Mercury (II) ion by Fluorescence Polarization via Gold Nanoparticle Enhancement. Angewandte Chemie International Edition 47, 8386-8389, 2008.

66. Bin-Cheng Yin, Honghua Li, and Bang-Ce Ye*. A Dual-Probe Hybridization Method for Reducing Variability in Single Nucleotide Polymorphism Analysis with Oligonucleotide Microarrays. Analytical Biochemistry 383, 270-278, 2008.

67. Bin-Cheng Yin, Honghua Li, and Bang-Ce Ye*. Microarray-based Estimation of SNP Allele-frequency in Pooled DNA using the Langmuir Kinetic Model. BMC Genomics 9, 605, 2008.

68. Bin-Cheng Yin, Fei Yue, and Bang-Ce Ye*. Construction of Microarrays for Genotyping of DQA using Unmodified 45-mer Oligonucleotide. Molecular Biotechnology 36, 142-150, 2007.

69. Bang-Ce Ye, Min Zhang, Bin-Cheng Yin. 英文专著《Nano-Bio Probe Design and Its Application for Biochemical Analysis》, ISBN 978-3-642-29542-3, 施普林格出版社，2012.